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Antimicrobial Effect of Polyphenols Enriched Fractions of Moringa oleifera Lam. Leaves at the Flowering Stage Against Microbial Strains Causing Oral and Wound Infections

Authors:

W. J. A. B. N. Jayasuriya ,

University of Sri Jayewardenepura, LK
About W. J. A. B. N.
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences
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N. D. Jayaweera,

University of Sri Jayewardenepura, LK
About N. D.
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences
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H. Adurapotha,

University of Sri Jayewardenepura, LK
About H.
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences
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F. D. Meedin,

University of Sri Jayewardenepura, LK
About F. D.
Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences
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D. I. Uluwaduge,

University of Sri Jayewardenepura, LK
About D. I.
Department of Basic Sciences, Faculty of Allied Health Sciences
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L. D. A. M. Arawwawala

Industrial Technology Institute, R & D Complex, Halbarawa, Thalahena, Malabe, LK
About L. D. A. M.
Herbal Technology Section
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Abstract

Purpose: Various parts of Moringa oleifera Lam. (Moringaceae) are used in traditional systems of medicine. This study investigates the antimicrobial activity of the crude aqueous extract and polyphenol enriched fractions of M. oleifera leaves at the flowering stage against microbial strains causing oral and wound infections.


Methods: Polyphenol enriched fractions (hydroalcoholic extract, methanol fresh leaves extract, methanol dry leaves extract and decoction) were prepared using four different methods and total polyphenolic content (TPC) and total flavonoid content (TFC) of the extracts were determined. Crude aqueous extract and the fractions were tested against ATCC reference strains of Escherichia coli and Candida albicans (oral pathogens) and Streptococcus aureus and Staphylococcus pyogenes (wound pathogens) using agar well diffusion method. Gentamycin and clotrimazole were used as the positive controls for bacteria and fungi respectively and distilled water was used as the negative control.


Results: Methanol dry leaves extract demonstrated highest values of TPC (11.92 ±0.39 mg GAE/g) and TFC (41.68±0.82 mg QE/g). Each polyphenol fraction demonstrated antibacterial activity against E. coli, S. aureus and S. pyogenes at concentrations of 500 - 1000 mg/ml. Hydro-alcoholic and methanol (fresh leaves) extracts demonstrated antifungal activity against C. albicans. The crude aqueous extract exhibited dose dependent antibacterial activity against S. aureus. All extracts demonstrated less antibacterial activity against S. pyogenes compared to S. aureus.


Conclusion: Polyphenol rich fractions of M. oleifera leaves at the flowering stage possessed antimicrobial activity against E. coli, S. aureus, S. pyogenes and C. albicans.

How to Cite: Jayasuriya, W.J.A.B.N., Jayaweera, N.D., Adurapotha, H., Meedin, F.D., Uluwaduge, D.I. and Arawwawala, L.D.A.M., 2021. Antimicrobial Effect of Polyphenols Enriched Fractions of Moringa oleifera Lam. Leaves at the Flowering Stage Against Microbial Strains Causing Oral and Wound Infections. Pharmaceutical Journal of Sri Lanka, 11(1), pp.27–40. DOI: http://doi.org/10.4038/pjsl.v11i1.70
Published on 31 Dec 2021.
Peer Reviewed

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