Research Papers
Formulation and evaluation of in vitro antacid effect of effervescent granules containing extracts of Evolvulus alsinoides
Authors:
W. J. A. B. N. Jayasuriya ,
University of Sri Jayewardenepura, LK
About W. J. A. B. N.
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences
H. U. V. Hettiarachchi,
University of Sri Jayewardenepura, LK
About H. U. V.
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences
T. D. M. T. S. Kumara,
University of Sri Jayewardenepura, LK
About T. D. M. T. S.
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences
N. T. B. Dias,
University of Sri Jayewardenepura, LK
About N. T. B.
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences
L. D. A. M. Arawwawala,
Industrial Technology Institute, Malabe, LK
About L. D. A. M.
Herbal Technology Section
T. S. Suresh
University of Sri Jayewardenepura, LK
About T. S.
Department of Biochemistry, Faculty of Medical Sciences
Abstract
Purpose: This study investigates the in-vitro gastroprotective activity of the aqueous and ethanolic extracts of Evolvulus alsinoides Linn plant and formulates an antacid dosage form subsequently.
Method: Extracts were prepared by refluxing and the gastroprotective activities were then evaluated by in-vitro neutralising of artificial gastric acid where the neutralising effect as well as the neutralising capacity were determined. The effervescent granular dosage form was formulated using the freeze-dried powder of the aqueous extract. In-vitro gastroprotection of the granules was evaluated using Vatier’s artificial stomach model in addition to the assays mentioned above. A commercially available antacid formulation (Eno) was used as the reference drug in each assay whereas distilled water and ethanol were used as the negative controls.
Results: Both aqueous and ethanolic extracts of E. alsinoides exert significant gastroprotection in acid neutralising effect and neutralising capacity assays (p<0.05). In-vitro gastroprotection given by the aqueous extract was significantly higher than the ethanolic extract (p<0.05). Formulated effervescent granules also resulted in significant acid neutralisation compared to distilled water in each in-vitro assay (p<0.001). Further, the neutralising capacity assay showed the concentration-dependent activity of the granules. Vatier’s stomach model which measures the duration of consistent acid neutralising, demonstrated the significant activity of the formulation (p<0.001).
Conclusion: E. alsinoides plants in aqueous and ethanolic extracts bear significantly higher in-vitro gastroprotective activities compared to respective negative controls (p<0.05). Effervescent granules formulated using E. alsinoides aqueous extract resulted a significant in-vitro gastroprotection in all the employed assay models.
How to Cite:
Jayasuriya, W.J.A.B.N., Hettiarachchi, H.U.V., Kumara, T.D.M.T.S., Dias, N.T.B., Arawwawala, L.D.A.M. and Suresh, T.S., 2022. Formulation and evaluation of in vitro antacid effect of effervescent granules containing extracts of Evolvulus alsinoides. Pharmaceutical Journal of Sri Lanka, 12(1), pp.9–19. DOI: http://doi.org/10.4038/pjsl.v12i1.84
Published on
30 Dec 2022.
Peer Reviewed
Downloads
