Research Papers
Evaluation of the in vitro anti-inflammatory activity of different fractions of the aqueous extract of Curcuma zedoaria Roscoe rhizome and formulation of a cream with anti-inflammatory potential
Authors:
K. G. Ileperuma,
General Sir John Kotelawala Defence University, Ratmalana, LK
About K. G.
Department of Pharmacy, Faculty of Allied Health Sciences
R. U. Senanayaka,
General Sir John Kotelawala Defence University, Ratmalana, LK
About R. U.
Department of Pharmacy, Faculty of Allied Health Sciences
H. P. D. De Silva,
General Sir John Kotelawala Defence University, Ratmalana, LK
About H. P. D.
Department of Pharmacy, Faculty of Allied Health Sciences
B. L. C. Samanmali,
General Sir John Kotelawala Defence University, Ratmalana, LK
About B. L. C.
Department of Pharmacy, Faculty of Allied Health Sciences
W. J. A. B. N. Jayasuriya,
University of Sri Jayewardenepura, Nugegoda, LK
About W. J. A. B. N.
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences
H. M. D. R. Herath
University of Sri Jayewardenepura, Nugegoda, LK
About H. M. D. R.
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences
Abstract
Introduction: Curcuma zedoaria Roscoe (C. zedoaria) is used in traditional medicine for various diseases, including inflammation. This study aimed to evaluate the in vitro anti-inflammatory activity of the aqueous extract of C. zedoaria rhizome and its fractions and develop an anti-inflammatory cream incorporating its aqueous extract.
Methods: The crude aqueous extract of C. zedoaria rhizome was prepared by the decoction method. A portion of it was freeze-dried and the other part was subjected to sequential fractionation using hexane, dichloromethane and ethyl acetate. All the fractions were dried using a rotary vacuum evaporator. The dry powders of the above extracts were assessed for their in vitro anti-inflammatory activity using heat-induced egg albumin denaturation assay and compared with the reference drug, diclofenac sodium. A cream was formulated by incorporating the most effective fraction; the aqueous extract and its anti-inflammatory activity was compared against a commercially available diclofenac sodium cream.
Results: The aqueous extract of C. zedoaria rhizome and its hexane, dichloromethane, ethyl acetate fractions and the remaining aqueous extract showed anti-inflammatory activity against heat-induced protein denaturation with the IC50 values; 94.45 µg/mL, 3822 µg/mL, 373.2 µg/mL, 337.7 µg/mL and 565.4 µg/mL respectively. The IC50 value obtained for diclofenac sodium powder was 915.7µg/mL. The IC50 value obtained for the formulated cream (containing 3% w/w aqueous extract of C. zedoaria rhizome) was 1894 µg/mL. The observed IC50 value of the commercially available diclofenac sodium 1% w/w cream was 1227 µg/mL.
Conclusion: The cream formulated with the aqueous extract of
C. zedoaria rhizome possesses
in vitro anti-inflammatory efficacy. Further studies are required to improve its drug-releasing capacity.
How to Cite:
Ileperuma, K.G., Senanayaka, R.U., De Silva, H.P.D., Samanmali, B.L.C., Jayasuriya, W.J.A.B.N. and Herath, H.M.D.R., 2022. Evaluation of the in vitro anti-inflammatory activity of different fractions of the aqueous extract of Curcuma zedoaria Roscoe rhizome and formulation of a cream with anti-inflammatory potential. Pharmaceutical Journal of Sri Lanka, 12(1), pp.20–32. DOI: http://doi.org/10.4038/pjsl.v12i1.85
Published on
30 Dec 2022.
Peer Reviewed
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